Factors Affecting Restriction Endonucleases Activity

Changes in ionic strength , divalent metal ions, pH, solvents, DNA methylation, temperature, star activity, and enzyme concentration influence the rate and the specificity of reactions catalyzed by restriction endonucleases. Buffer composition Different restriction enzymes have differing preferences for ionic strengths (salt concentration) and cations (Na+ or K+). Major function of the buffer is to … Read More