Centrifugation is a technique, used for separation of particles including macromolecules by sedimenting them under varying gravitational force. Different particles of different masses settles in a tube at different rates in response to gravity. Centrifugal field applied by a centrifuge increases the settling rate of particles causing their separation. This technique is valuable for determination … Read More


Spectrophotometry is a quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. This  depends upon how much light is absorbed by a chemical substance. Measurement is done by recording the intensity of light absorbed as a beam of light passes through sample solution. A spectrophotometer is an instrument … Read More

Chemical Separation Techniques

Chemical separation techniques play a key role in analyzing molecules or compounds used for biological, physical, and chemical research. They are a powerful tool set to investigate mechanisms of various biological processes. It is used to separate a mixture into its two or more distinct components, which are used further for the analysis of physical … Read More


Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor through a medium in which, the components move at different rates. It involves a sample being dissolved in a mobile phase (which may be a gas, a liquid or a supercritical fluid). Then, mobile phase is then … Read More