Molecular biology is the field of biology (Figure 1) that studies the interactions between the various systems of a
Figure 1. Molecular biology lab
This interactions include the interrelationship of
DNA, RNA and protein synthesis and carry out the biological processes essential for the cell functions and maintenance.
Restriction enzymes are also called ‘molecular scissors’ as they cleave DNA at or near specific recognition sequences known as restriction sites.
These enzymes make one incision on each of the two strands of
DNA. They are also called restriction endonucleases.
Molecular genetic testing, most of which are different from the uses of traditional clinical laboratory testing and even molecular biologic testing in diseases, such as, infectious disease, cancer etc.
Types of Techniques
Various techniques/steps are used in genomics are shown below:
DNA isolation Separation of
DNA Cutting and joining of
DNA Cloning with vectors
Detection of gene of interest
DNA and cloning Production of copies of
DNA using polymerase chain reaction (PCR)