Electrophoresis is defined as a technique, where the migration and separation of charged particles (ions) occur under the influence of an electric field.
The process does not affect the molecular structure of compounds being separated, yet it is highly sensitive to differences in molecular charge and mass of the components of the sample (Figure 1).
This course deals with various types, general principles, procedures, and applications of electrophoresis in various fields.
Applications of Electrophoresis
Electrophorectic separation techniques are applied in clinical investigation, biochemical research, pharmacology, biology, food, and forensic science (Figure 2). It is a highly analytical tool.
It is also used for isolating biological factors, which are associated with diseases, such as cystic fibrosis, sickle-cell anemia, myelomas, and leukemia.